From owner-diagnostics@net.bio.net Wed Apr 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Bryan Kiehl Newsgroups: bionet.diagnostics Subject: Re: Shelf life of ELISA reagents Date: 1 May 1997 01:23:04 -0700 Organization: GenBio Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5k9jt8$22u@net.bio.net> NNTP-Posting-Host: net.bio.net I think you may want to consider more than stability. Contamination, usually microbial type, of materials can participate in "background". If not taken into account, you may also consider plastic changes, assuming ELISA mean microtiter plates. On 30 Apr 1997 04:06:22 -0700, "D.A.C. Jones" wrote: >Does any body have any thoughts on the useful shelf life of ELISA >reagents. I've recently been doing a series of experiments comparing the >sensitivity of various ELISA protocols. One thing I have noticed, which >is slightly annoying, is that on opening a new tin of skimmed milk >(Marvel) and a new bottle of tween-20 (BDH) there are big reductions in >the background. OK, so this may have been expected, but not to the extent >that I have noticed. Both of the 'old' reagents had probably been opened >less than 6 months ago. Any tips on storage etc? > > >Regards >Dave Bryan Kiehl GenBio, San Diego BKiehl@GenBio.com .