From owner-drosophila@net.bio.net Mon Oct 09 23:00:00 1995
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!elvis.delphi.com!uunet!in2.uu.net!noc.near.net!das-news2.harvard.edu!oitnews.harvard.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!cis.ohio-state.edu!nntp.sei.cmu.edu!bb3.andrew.cmu.edu!andrew.cmu.edu!sc6c+
From: "Sidney B. Cambridge" <sc6c+@andrew.cmu.edu>
Newsgroups: bionet.drosophila
Subject: How to fix injected embryos ?
Date: Tue, 10 Oct 1995 11:12:57 -0400
Organization: Doctoral student, Biology, Carnegie Mellon, Pittsburgh, PA
Lines: 11
Message-ID: <4kScptS00iV3Q3AY0u@andrew.cmu.edu>
NNTP-Posting-Host: po7.andrew.cmu.edu


I am in the process of learning how to fix and antibody-stain injected
embryos. All of the protocols I found call for fixation of the embryos
while being attached to the coverslip. Besides wondering if there isn't
any other way of fixing, I can't figure out how exactly the coverslip is
placed in a biphasic solution 
(PEM/heptane) ????? Can anybody help me ?
Thanx in advance.

Cheers,
Sidney   (sc6c@andrew.cmu.edu)

.