From owner-diagnostics@net.bio.net Tue Apr 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "D.A.C. Jones" Newsgroups: bionet.diagnostics Subject: Shelf life of ELISA reagents Date: 30 Apr 1997 04:06:22 -0700 Organization: Scottish Crop Research institute Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5k793e$6d8@net.bio.net> NNTP-Posting-Host: net.bio.net Does any body have any thoughts on the useful shelf life of ELISA reagents. I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago. Any tips on storage etc? Regards Dave .