From owner-cellbiol@net.bio.net Thu Feb 27 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!xfer.kren.nm.kr!news-xfer.netaxs.com!news.fast.net!uunet!in3.uu.net!204.194.12.17!avalanche.ixa.net!ixa.net!news.pacificrim.net!news From: Jim Kling Newsgroups: bionet.cellbiol,bionet.general,bionet.microbiology,bionet.immunology,sci.bio.misc,sci.bio.technology,sci.med Subject: labeling systems Date: Fri, 28 Feb 1997 14:56:37 +0000 Organization: Pacific Rim Network, Inc. Lines: 41 Distribution: inet Message-ID: <3316F225.45C8@nasw.org> Reply-To: jkling@nasw.org NNTP-Posting-Host: whatcom-ppp47.pacificrim.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) Xref: biosci bionet.cellbiol:6803 bionet.general:25821 bionet.microbiology:9132 bionet.immunology:11069 sci.bio.misc:7524 sci.bio.technology:7071 sci.med:152437 Hi all- I posted a message a couple of months ago soliciting opinions for an article that I was writing for The Scientist (http://www.the-scientist library.upenn.edu/) on centrifuges. That worked out so well I decided to try it again. This time, I'm writing a similar article on molecular labeling systems. The article will be a survey of products available (by no means complete, but hopefully representative). I'd like to get user comments about: 1) What kinds of labeling systems are best suited to what kinds of research? (ie, DNA sequencing, transcription studies, chromosomal imaging, etc). Don't neglect to mention something because you think its obvious; keep in mind that the readers of these articles are often purchasing managers who may not be intimately familiar with research uses (I may not be either, for that matter). 2) What advantages and disadvantages does each labeling system have (ie cost, sensitivity, ease-of-use, etc). Which of these properties is most important to you? Least important? 3) Are there specific deficiencies in current labeling technology? (ie: It would be really great if labeling systems were capable of...) 4) What are the trends in labeling sytems? Are there new approaches that are gaining popularity, older ones that are fading away? Please respond to jkling@nasw.org, and include your full name and title (ie, molecular biologist, geneticist, etc) and university/company affiliation so I can quote you appropriately. Thanks! -- Jim Kling science/medical writing Bellingham, WA jkling@pacificrim.net http://nasw.org/users/jkling .