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From: A Giessauf <ldltryptophan@hotmail.com>
Newsgroups: bionet.diagnostics
Subject: Virus purification project - search for partners
Organization: Karl-Franzens-Universitaet Graz
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Date: Thu,  8 Jan 2004 14:08:31 +0000 (GMT)
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      Dear all,

      I am about starting to write a proposal for a research project

      about a novel method for isolation / purification of virus

      particles from cell culture supernatants for use as antigens for

      diagnostic assay production.

      I have published the first article on this method "A technique for

      isolation of rubella virus-like particles by sucrose gradient

      ultracentrifugation using Coomassie brilliant blue G crystals" last

       year (Anal.Biochem. 308 (2002) 232-238) and I am searching now

       for

       (1) interested international research partners, who can provide me

       with stable transfected cell lines (or other expression systems)

       that secretes any virus-like particles that may become by my

       research more interesting for use as an antigen for assay

       production. I will try to carry forward the technique from the

       already described method for Rubella virus purification also to

       other virus particles.

       Density gradient ultracentrifugation has to be the final

       purification step.

       (2) Any industrial research partners that are interested to test

        antigens for assay production (e.g. immunoblot test stripes) are

        also welcome and will be mentioned in my research proposal.

        The advantage for an industrial partner in such a collaboration

        will be to obtain antigens at a lower price and the greatest part

        of the developing work will be done by my research work.


			Please contact my as soon as possible if you are

				interested ,

                            any comments or ideas are welcome.


			Sincerely yours,

                                     Andreas Gießauf, PhD
                                     andreas.giessauf@uni-graz.at




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