From owner-dros@hgmp.mrc.ac.uk Fri Dec 14 13:30:25 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BF8D917B14 for ; Fri, 14 Dec 2001 13:30:21 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 52BE017B1F for ; Fri, 14 Dec 2001 13:30:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id E75B717B19; Fri, 14 Dec 2001 13:30:04 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id EA52517AEE for ; Fri, 14 Dec 2001 00:27:23 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 72DB017A86; Fri, 14 Dec 2001 00:27:22 +0000 (GMT) To: bionet-drosophila@net.bio.net From: phopkins@gladstone.ucsf.edu (Paul Hopkins) Newsgroups: bionet.drosophila Subject: Yellow-green flies??? Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1248717A87 for ; Fri, 14 Dec 2001 00:27:19 +0000 (GMT) X-Received: from gladstone.ucsf.edu (gladstone.ucsf.edu [128.218.162.25]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 15BC017A86 for ; Fri, 14 Dec 2001 00:27:18 +0000 (GMT) X-Received: from [128.218.162.97] (128.218.162.97) by gladstone.ucsf.edu with ESMTP (Eudora Internet Mail Server 3.0.3) for ; Thu, 13 Dec 2001 16:36:07 -0800 X-To: dros@net.bio.net Date: Fri, 14 Dec 2001 13:30:04 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I'm relatively new to drosophila, and have been doing RNAi for a gene of unknown function using a UAS-inverted repeat construct. I see late eclosure, but possibly more interesting is a pale yellow-green colour in newly eclosed males. It is particularly noticeable on the ventral side of the abdomen, but is no longer noticeable after about 12 hours. It is more yellow in colour than the jade-green stuff that newly eclosed flies secrete. This colour occurs only in males and in none of the control flies, male or female. Before I go overboard trying to identify what this yellow-green stuff is: has anyone seen anything like this before??? Being new to flies, I don't want to be chasing a red-herring... thanks! Paul PS please cc me if you reply to this. --- .