From owner-cellbiol@net.bio.net Thu Feb 27 22:00:00 1997
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From: ziping@u.washington.edu (Ziping Yang)
Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol
Subject: How to separate vegf dimer into monomer?
Date: Fri, 28 Feb 1997 09:33:35 -0700
Organization: University of Washington
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Hi, everybody
I run vegf western and use B-mercaptoethanol (freshly added to lysis
buiffer at 280-400 mM) as a reducing reagent. Samples were boiled before
loading to gel. However,  VEGF dimer is always there and showed much
strong band than monomer did. Any suggestions are wellcomed.
ziping

.