From owner-cellbiol@net.bio.net Fri Jan 31 22:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!news-peer.gsl.net!news.gsl.net!news-hk.gsl.net!news.gsl.net!newsgate.cuhk.edu.hk!news.cuhk.edu.hk!usenet From: Rocky Ho Newsgroups: bionet.cellbiol Subject: Freezing Cell Date: Sun, 02 Feb 1997 03:54:11 +0800 Organization: The Chinese University of Hong Kong Lines: 8 Message-ID: <32F39F63.4D46@cuhk.edu.hk> NNTP-Posting-Host: @dialup061.csc.cuhk.edu.hk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) Dear collegues, I am now going to freeze hepatocyte culture. I encounter two suggestion for the storage medium. One is Culture medium with DMSO and the other is FCS with 10% glycerol. Does anyone has idea on the benefit of using culture medium or FCS as storage medium? Rocky Ho .