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To: bionet-drosophila@net.bio.net
From: rjd23@cus.cam.ac.uk ("R.J. Davenport")
Newsgroups: bionet.drosophila
Subject: plasma membrane targeting sequence
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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Date: Wed, 20 Jun 2001 15:10:17 +0100 (BST)
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hi,
I am trying to express plant ion channels in S2 cells. I have constructs
with C-terminal GFP tags and have determined that the gene products are
targeted to endomembranes in S2. I want to patch-clamp transformed cells,
so I need plasma membrane-expressed proteins. Can anyone suggest a
reliable PM targeting sequence in Drosophila?
Thanks very much!
Romola

Romola Davenport
Dept of Plant Sciences
Downing St Cambridge CB2 3EA
(01223) 330 210 (lab)*
Newnham College
Cambridge CB3 9DF
(01223) 335 810*
*omit first '3' if using an internal line




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