From owner-dros@hgmp.mrc.ac.uk Tue Dec 3 14:48:02 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 225A67D0C5 for ; Tue, 3 Dec 2002 14:48:02 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9C0197D0F8 for ; Tue, 3 Dec 2002 14:48:01 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 2817B7D0C5; Tue, 3 Dec 2002 14:48:01 +0000 (GMT) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BAC7A7D191 for ; Tue, 3 Dec 2002 00:55:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 4F0E77D0CA; Tue, 3 Dec 2002 00:55:14 +0000 (GMT) To: bionet-drosophila@net.bio.net From: fisherm@u.washington.edu (Michelle Fisher) Newsgroups: bionet.drosophila Subject: early embryo GFP fix Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-version: 1.0 Content-type: text/plain; charset="US-ASCII" Content-transfer-encoding: 7bit X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id EF9EF7D191 for ; Tue, 3 Dec 2002 00:55:11 +0000 (GMT) X-Received: from mxout1.cac.washington.edu (mxout1.cac.washington.edu [140.142.32.134]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8F3E27D0CA for ; Tue, 3 Dec 2002 00:55:10 +0000 (GMT) X-Received: from mailscan-out2.cac.washington.edu (mailscan-out2.cac.washington.edu [140.142.33.17]) by mxout1.cac.washington.edu (8.12.1+UW01.12/8.12.1+UW02.11) with SMTP id gB30t3VM026040 for ; Mon, 2 Dec 2002 16:55:08 -0800 X-Received: FROM mailhost2.u.washington.edu BY mailscan-out2.cac.washington.edu ; Mon Dec 02 16:54:59 2002 -0800 X-Received: from [128.95.253.218] (D-128-95-253-218.dhcp4.washington.edu [128.95.253.218]) by mailhost2.u.washington.edu (8.12.1+UW01.12/8.12.1+UW02.11) with ESMTP id gB30sxqk000746 for ; Mon, 2 Dec 2002 16:54:59 -0800 X-User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.0.6 X-To: Date: Tue, 3 Dec 2002 14:48:01 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk I was wondering if anyone has a protocol for an early embryo vitelline membrane permeablizing fix that preserves a GFP signal? Thanks for your help Michelle ----------------------------------- Michelle Fisher Department of Zoology University of Washington Box 351800 --- .