From owner-celegans@net.bio.net Sun Jun 16 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!elroy.jpl.nasa.gov!usc!newshub.csu.net!newshub.sdsu.edu!info.ucla.edu!nnrp.info.ucla.edu!news.ucdavis.edu!bullwinkle.ucdavis.edu!not-for-mail From: szmazer@bullwinkle.ucdavis.edu (Jonathan Mazer) Newsgroups: bionet.molbio.proteins,bionet.celegans Subject: Re: help with a biochemistry lab Followup-To: bionet.molbio.proteins,bionet.celegans Date: 17 Jun 1996 04:41:45 GMT Organization: University of California, Davis Lines: 18 Message-ID: <4q2nm9$c3a@mark.ucdavis.edu> References: NNTP-Posting-Host: bullwinkle.ucdavis.edu Xref: biosci bionet.molbio.proteins:8144 bionet.celegans:947 Dr. Wightman, Here at U.C. Davis, Lactate Dehydrogenase was purified in one step from beef steak (or heart muscle) using a NADH affinity column. The assay was, of course, NADH to NAD+ monitoring absorbance at 340 nm. It took one or two 5 hour lab periods. It was very simple and it taught them purification using a Waring blender, affinity chromatography, fraction collectors, spectrophotometer, and all about glycolysis. The experiment was done in ice buckets and a cold room. Each lab had 30 students working in pairs. (I am talking about the undergraduate biochemistry lab class, formerly known as 101L. It has a new number, but I don't know it) I was a T.A. years ago and they might have changed the lab by now, but you can contact Dr. Larry Sprechman in Biochemistry and he can provide you with all the information. We are also only an hour away from you, so you come up here to see the set-up for yourself. Good Luck, Jonathan Mazer, PhD candidate. .