From owner-drosophila@net.bio.net Wed Feb 23 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!post.its.mcw.edu!news.doit.wisc.edu!avery.genetics.wisc.edu!user From: wrengels@facstaff.wisc.edu (Bill Engels) Newsgroups: bionet.drosophila Subject: Re: PCR alternative to plasmid rescue? Followup-To: bionet.drosophila Date: Thu, 24 Feb 1994 14:12:35 -0600 Organization: University of Wisconsin Lines: 36 Message-ID: References: <1994Feb21.114121.1@eagle.wesleyan.edu> NNTP-Posting-Host: avery.genetics.wisc.edu >apparently some workers have >utilized a PCR technique, involving digestion of genomic DNA, circularization >of the resulting fragments, and amplification using cassette-specific primers, >which point away from each other, to amplify genomic DNA flanking the >P-element cassette. The technique you refer to is called inverse PCR. Here are a couple of references: Ochman, H., A. S. Gerber and D. L. Hartl, 1988 Genetic applications of an inverse polymerase chain reaction. Genetics 120: 621-623. Gloor, G. B. and W. R. Engels, 1992 Single fly DNA preps for PCR. Dros. Inform. Ser. 71: 148-149. > Specifically, I need pCaSpeR sequence information We have pieced together a CaSpeR sequence, and I will send it by e-mail to you or anyone else who is interested. By the way, what is a ÒP-element cassetteÓ? Is that the same as a P element? Cheers, __ ______ /5' \\ /X\ /X\ /X\ /X\ /X\ /X\ / Bill \ /X\ /X\ /X\ /X\ /X \X/ \X/ \X/ \X/ \X/ \X/ \X_______ __X/ \X/ \X/ \X/ \X/ \3' ----------------------------------------------------------------------------- William R. Engels : WREngels@macc.wisc.edu Genetics Department, 445 Henry Mall : office: (608) 263-2213 University of Wisconsin : lab: (608) 262-5578 Madison, WI 53706 : FAX: (608) 262-2976 ----------------------------------------------------------------------------- .