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From: Kelly Beumer <kelly@biochem.utah.edu>
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Hi, Tony,
I am thinking about making gateway vectors as well, and saw that 
Terry Murphy at the Carnegie institute has made a few that will be 
available to the community soon. Did you get any input on this note 
you posted last october on flybase?

ref: news.indiana.edu bionet.drosophila:148

Fly gurus,
         We're wondering if there is any experience using p element
transformation vectors (for example pUAST) made by gateway cloning
(invitrogen).   Is there any difference in expression or mobilization
from a p-element having the 20-odd basepair recombination site
internally in the polylinker?  We are making  p-element transformation
plasmids this way, but would like to know if we are getting ourselves
into trouble.

Thanks,

Thanks for lettling me know!

-- 
Kelly Beumer
Biochemistry Department
University of Utah
20 N. 1900 East, Rm. 211
Salt Lake City , UT 84132-3201

1-801-581-3848

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<div>Hi, Tony,</div>
<div>I am thinking about making gateway vectors as well, and saw that
Terry Murphy at the Carnegie institute has made a few that will be
available to the community soon. Did you get any input on this note
you posted last october on flybase?</div>
<div><br></div>
<div><font face="Charcoal" color="#000000">ref: news.indiana.edu
bionet.drosophila:148<br>
<br>
Fly gurus,<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; We're wondering if there is
any experience using p element<br>
transformation vectors (for example pUAST) made by gateway cloning<br>
(invitrogen).&nbsp;&nbsp; Is there any difference in expression or
mobilization<br>
from a p-element having the 20-odd basepair recombination site<br>
internally in the polylinker?&nbsp; We are making&nbsp; p-element
transformation<br>
plasmids this way, but would like to know if we are getting
ourselves<br>
into trouble.</font><br>
</div>
<div><font face="Charcoal" color="#000000">T</font><font
color="#000000">hanks,</font></div>
<div><font color="#000000"><br></font></div>
<div><font color="#000000">Thanks for lettling me know!</font></div>
<div><br></div>
<x-sigsep><pre>-- 
</pre></x-sigsep>
<div>Kelly Beumer<br>
Biochemistry Department<br>
University of Utah<br>
20 N. 1900 East, Rm. 211<br>
Salt Lake City , UT 84132-3201<br>
<br>
1-801-581-3848</div>
</body>
</html>

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