From owner-cellbiol@net.bio.net Wed Jan 08 22:00:00 1997 Path: biosci!news.artemis.com!uunet!in3.uu.net!169.132.11.200!news.idt.net!enews.sgi.com!news.sgi.com!sdd.hp.com!night.primate.wisc.edu!ames!purdue!oitnews.harvard.edu!news.dfci.harvard.edu!mbcrr!york From: york@mbcrr.dfci.harvard.edu (Ian A. York) Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol Subject: Enzyme assay for ER permeability Date: 9 Jan 1997 20:32:42 GMT Organization: Dana-Farber Cancer Institute Lines: 19 Message-ID: <5b3kla$sd6@netman-mel.dfci.harvard.edu> NNTP-Posting-Host: mbcrr.harvard.edu Xref: biosci bionet.molbio.methds-reagnts:53293 bionet.cellbiol:6380 I want to be able to measure the degree of permeablization of the plasma membrane and compare it to the degree of permeablization of the ER membrane. To look at plasma membrane permeability, I can assay for lactate dehydrogenase; what's an easily-measured enzyme (or, more generally, what's a simple assay--it doesn't have to be enzymatic) that will specifically measure ER membrane permeability? For that matter, does anyone have an assay for plasma membrane permeability that they feel is simpler and/or more informative than LDH? (Trypan blue won't cut it; for one thing, I need to know about loss of proteins much larger than trypan blue.) Any answers or guesswork appreciated. Thanks. Ian -- Ian York (york@mbcrr.harvard.edu) "-but as he was a York, I am rather inclined to suppose him a very respectable Man." -Jane Austen, The History of England .