From owner-cellbiol@net.bio.net Wed Jan 29 22:00:00 1997 Path: biosci!rutgers!news-relay.ncren.net!newz.oit.unc.edu!mac295a6013.med.unc.edu!user From: jek@med.unc.edu (Jackie E. Kylander) Newsgroups: bionet.cellbiol Subject: Re: Freezing Cells Date: Thu, 30 Jan 1997 08:58:24 -0500 Organization: UNC-Chapel Hill Lines: 29 Message-ID: References: <32EFA422.5F3@pi.net> NNTP-Posting-Host: mac295a6013.med.unc.edu In article <32EFA422.5F3@pi.net>, Jack JWA van Loon wrote: >Does any of you have any experience in freezing (and storing) single >cells at -20 dgr.C ? >What happens if a cell suspension (with DMSO) goes trough a temperature >profile of -10 to -70 degr.C? >Do you know of any references ....? They are very hard to find. > >Thank you in advance, > >Jack van Loon >Gorinchem, The Netherlands >jvanloon@pi.net Jack, contact Nalge (Europe) Limited, Foxwood Court, Rotherwas, Hereford, HR2 6JQ, United Kingdom, Tel: (0432)263933 , and ask for their Cryopreservation Manual. It's only about 9 pages long and doesn't go into great detail but is well written and covers most of your questions including freezing rates and storage temperatures recommended for various cell types. While Nalge publishes this, it was written by Frank Simione of the American Type Culture Collection and is NOT an advertisement for their products. -- Jackie E. Kylander A/OSB MAG TCS Mac Nanny .