From owner-biophys@hgmp.mrc.ac.uk Sun Apr 29 13:33:24 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9944217B52 for ; Sun, 29 Apr 2001 13:33:22 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3FE2517AC0; Sun, 29 Apr 2001 13:33:18 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B921117B4E; Sun, 29 Apr 2001 13:33:12 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id NAA07532; Sun, 29 Apr 2001 13:33:11 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Mavrommatis Kostas" X-Newsgroups: bionet.biophysics,bionet.general,bionet.molec-model Subject: Salt bridges Date: 29 Apr 2001 13:33:10 +0100 Organization: National Technical University of Athens, Greece Lines: 11 Message-ID: <9ch1lu$4j6$1@ulysses.noc.ntua.gr> X-Complaints-To: news@net.bio.net X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.3018.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.3018.1300 To: bioforum@hgmp.mrc.ac.uk, biophys@hgmp.mrc.ac.uk, molmodel@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk Hi everybody, does anybody knows if there is an experimental method for identifying the formation of a salt bridge, apart from structural studies? I have some mutants of a protein that are supposed to form different number of salt bridges and I want to verify this. Is there a way to do this by using chaotropic salts or DSC? or something else? I thank you in advance. .