From owner-dros@hgmp.mrc.ac.uk Sat Oct 23 17:17:44 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 0014F7D0C1 for ; Sat, 23 Oct 2004 17:17:43 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8227F7D0C6 for ; Sat, 23 Oct 2004 17:17:43 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 9882B7D0C1; Sat, 23 Oct 2004 17:17:40 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C62497D0F8 for ; Fri, 22 Oct 2004 18:47:17 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 6E56E7D0C4; Fri, 22 Oct 2004 18:47:15 +0100 (BST) To: bionet-drosophila@net.bio.net From: Man.Mohan@gen.bio.uni-giessen.de (Man Mohan) Newsgroups: bionet.drosophila Subject: dsRNAi positive control Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: <20041022173157.F34F4304C65@hermes2.hrz.uni-giessen.de> Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit MIME-Version: 1.0 X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CE5507D0F8 for ; Fri, 22 Oct 2004 18:47:13 +0100 (BST) X-Received: from merkurneu.hrz.uni-giessen.de (merkurneu.hrz.uni-giessen.de [134.176.2.3]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 2A7867D0C4 for ; Fri, 22 Oct 2004 18:47:11 +0100 (BST) X-Received: from [134.176.2.168] by merkurneu.hrz.uni-giessen.de with ESMTP for dros@net.bio.net; Fri, 22 Oct 2004 19:31:55 +0200 X-Received: from popg.uni-giessen.de (jobim.hrz.uni-giessen.de [134.176.3.137]) by hermes2.hrz.uni-giessen.de (Postfix) with ESMTP id F34F4304C65 for ; Fri, 22 Oct 2004 19:31:57 +0200 (CEST) X-User-Agent: IMHO/0.98.3 (Webmail for Roxen) X-To: dros@net.bio.net X-HRZ-JLUG-MailScanner-Information: Passed JLUG virus check X-HRZ-JLUG-MailScanner: Found to be clean Date: Sat, 23 Oct 2004 17:17:40 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi I am planning to use dsRNA to silence my gene of interest in Drosophila S2 cell line. I am wondering which positive control should I use. I dont have an S2 cell line having integration of luciferase or GFP. Also, I do have an antibody for my gene of interest, but would go for first for RT-PCR as I dont have any other common antibody to see the expression of a given positive control. By the way, I expect that the dsRNA to my gene shall arrest the cell cycle. Your suggestions and help will be appreciated. man mohan Institute for Genetics Justus-Liebig University Heinrich-Buff-Ring 58 35392 Giessen Germany --- .