From owner-dros@hgmp.mrc.ac.uk Tue Jun 19 22:40:30 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 801E117AC0 for ; Tue, 19 Jun 2001 22:40:27 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3099A17B75 for ; Tue, 19 Jun 2001 22:40:20 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 1ED7617B79; Tue, 19 Jun 2001 22:39:38 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3B7DA17B3A for ; Tue, 19 Jun 2001 13:49:55 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id C796C17B47; Tue, 19 Jun 2001 13:49:30 +0100 (BST) To: bionet-drosophila@net.bio.net From: vlloyd@IS.Dal.ca (Vett Lloyd) Newsgroups: bionet.drosophila Subject: inducible expression vector for S2 cells Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 34B1317B3E for ; Tue, 19 Jun 2001 13:49:25 +0100 (BST) X-Received: from Lucy.UCIS.Dal.Ca (Lucy.UCIS.Dal.Ca [129.173.1.40]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E390517B10 for ; Tue, 19 Jun 2001 13:49:05 +0100 (BST) X-Received: from [129.173.16.209] (VLloyd-3.Biology.Dal.Ca [129.173.16.209]) by Lucy.UCIS.Dal.Ca (8.10.2/8.10.1) with ESMTP id f5JCn4t01189 for ; Tue, 19 Jun 2001 09:49:04 -0300 (ADT) X-To: dros@net.bio.net Date: Tue, 19 Jun 2001 22:39:38 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Dear Colleagues, Can anyone recommend a source or supplier for a vector that allows transient (or stable) inducible expression in S2 cells which does not use the metallothionein promotor. A heat shock promotor or similar would be ideal. Thank you in advance, Vett Lloyd -- Dr. Vett Lloyd Dept. Biology Dalhousie University Life Sciences Building 1355 Oxford St. Halifax, Nova Scotia, B3H 4J1, Canada phone (902) 494 2551 FAX (902) 494 3736 http://is.dal.ca/~biology2/faculty/lloyd/index.htm --- .