From owner-diagnostics@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU Newsgroups: bionet.diagnostics Subject: postdoc Date: 22 May 1995 13:13:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <950522052659.20200704@LIFSCI.SDSU.EDU> NNTP-Posting-Host: net.bio.net PLEASE POST Postdoctoral Fellowship Salmonella genome May 22nd 1995 Dear colleague, The primary postdoc on our Salmonella project has been offered his own laboratory at DOE/WSU. I am thus looking for someone to take over his position as soon as possible. If you know someone who might be interested I would be grateful if you could direct that person to me by e-mail to McClelland@lifsci.sdsu.edu or by FAX to 619 535 5472. The first step of this project is well advanced with 95% of the genome covered by an order and overlapping set of lambda clones. Approximately, 20% of these have been restriction mapped. We have delineated in these clones many of the known loops that distinguish Salmonella from E. coli. The most interesting part of the project is just starting in which we delineate the role of some of these lifestyle-specific regions. The next stage of the project will also involve the use of RNA fingerprinting by arbitiarily primed PCR in identify coordinately regulated genes in point mutations and large deletions in these regions (Wong K.K. and McClelland M. 1994. A stress-induced gene from Salmonella typhimurium identified by arbitrarily primed PCR of RNA (RAP). Proc. Natl. Acad. Sci. U.S.A. 91:639- 643; McClelland et al., Trends in Genetics, June 1995). Se also Wong K.K. and McClelland M. 1994. High-resolution restriction map for a 240- kilobase region spanning 91- to 96 minutes on the Salmonella typhimurium LT2 genome. J. Bacteriol. 176: 5729-5734. I am seeking someone with leadership and organizational skills who will coordinate with our collaborator Ken Sanderson and will direct a technician on the project. I would greatly appreicate your help in locating such an individual. Michael McClelland Director, Cal. Inst. Biol. Research 11099 North Torrey Pines Road La Jolla CA 92037 Phone 619 535 5486 FAX 619 535 5472 E-mail McClelland@lifsci.sdsu.edu .