From owner-bionews@hgmp.mrc.ac.uk Wed Jul 19 03:45:17 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 9220317AE9; Wed, 19 Jul 2000 03:45:15 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6022) id 7FC6B17A6D; Wed, 19 Jul 2000 03:45:14 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 4249617A96; Tue, 18 Jul 2000 02:25:55 +0100 (BST) Received: from rutgers.rutgers.edu (bsd.rutgers.edu [165.230.4.71]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 561CB415CF for ; Tue, 18 Jul 2000 02:25:54 +0100 (BST) Received: from newsmonger.rutgers.edu (newsmonger.rutgers.edu [165.230.4.67]) by rutgers.rutgers.edu (8.8.8/8.8.8) with SMTP id VAA18423 for ; Mon, 17 Jul 2000 21:25:53 -0400 (EDT) Received: (qmail 13515 invoked by uid 21); 18 Jul 2000 01:25:30 -0000 To: bionet-announce@rutgers.rutgers.edu From: gfp-1 Newsgroups: bionet.announce Subject: Green Fluorescent Protein Purification Short Course Organization: The Center for the study of Bioluminescence and Biotechnology Rutgers University Cook College Message-ID: <3973B2CE.35E@rci.rutgers.edu> Reply-To: meton@rci.rutgers.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: newsmonger.rutgers.edu 963883530 12648 165.230.209.152 (18 Jul 2000 01:25:30 GMT) X-Complaints-To: news_support@email.rutgers.edu NNTP-Posting-Date: 18 Jul 2000 01:25:30 GMT X-Mailer: Mozilla 3.0 (Macintosh; U; PPC) Date: Wed, 19 Jul 2000 03:45:14 +0100 (BST) Sender: owner-bionews@hgmp.mrc.ac.uk Precedence: bulk I have been asked to announce the following, The State University of New Jersey Rutgers Campus at New Brunswick Center for Research and Education in Bioluminescence and Biotechnology Programs in Biotechnology, Presents: Protein Purification: Isolation, Analysis, and Characterization of GFP, A Five and One-Half Day Hands-On Laboratory Course Using the remarkable Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression, as the source material July 23-28, 2000 and July 30-August 4, 2000 More than 600 scientists from around the world have strongly recommended this intensive course as an opportunity to develop protein research and analytical skills in a retreat setting. Participants work hard, identify and solve problems in the lab and enjoy camaraderie and good food and beer with colleagues. This five and one-half day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments. A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP*), which will be purified from an exceedingly crude form to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and capillary zone electrophoresis. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match. A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course. *Note: The use of GFP from a recombinant source (E. coli) is also being used as starting material due to its popularity within the scientific community. For further details you can reach us, by E-mail at: meton@rci.rutgers.edu by phone at: (732) 932-9071 extension 225 by FAX at: (732) 932-3633 for a brochure and further information please visit the GFP purification short course official Web site at: http://www.rci.rutgers.edu/~meton/protein.html Notice: We will be discounting the registration fee by 20% for anyone registering via the WEB! .