From owner-drosophila@net.bio.net Wed Feb 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!usenet From: Andrea Marie Peier Newsgroups: bionet.drosophila Subject: EMS vs P-element screen Date: Wed, 07 Feb 1996 23:41:35 -0600 Organization: Baylor College of Medicine, Houston, Tx Lines: 7 Message-ID: <31198D0F.2781E494@bcm.tmc.edu> NNTP-Posting-Host: kiwi.imgen.bcm.tmc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-User: ap691301 X-Mailer: Mozilla 2.0 (X11; I; SunOS 4.1.3_U1 sun4m) I'm a second year graduate student working on my PhD qualifying exam. I am proposing to do a genetic screen in Drosophila and am unsure about my choice of mutagen. Could someone please give me a few pros and cons regarding P-element vs EMS mutant screens? (I am screening for enhancers/suppressors of a particular mutant phenotype) Thanks .