From owner-celegans@net.bio.net Fri Oct 18 23:00:00 1996
Path: biosci!JHUVMS.HCF.JHU.EDU!hutter
From: hutter@JHUVMS.HCF.JHU.EDU (Harald Hutter)
Newsgroups: bionet.celegans
Subject: Tc1 STS mapping
Date: 19 Oct 1996 08:56:01 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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I would like to use the Tc1 STS mapping strategy for mapping mutants.
Therefore a few questions to get up to date.

1. Is there a common source for the primers now?
2. Has anyone extended the original set of markers provided by Ben Williams
and coworkers, e.g. included some of the newly mapped Tc1 sites from the
Plasterk lab?
3. Is the map position of the markers in the methods books up to date or
has the position of any of the markers changed since then. (Are the
insertion points in Acedb? I couldn't find them.)
4. Has anyone changes/improvements for the procedure?
5. What are typical problems/critical parameters in newly implementing this
technique?
Out of curiosity: Why the markers are spaced so closely that you need to
run a acrylamid gel to separate them?

Thanks for your help.

Harald

Harald Hutter                  Hedgecock lab

Johns Hopkins University
Department of Biology
Room 7, Mudd Hall              phone   +1 410 516 8381
3400 N. Charles Street         fax     +1 410 516 5213
Baltimore, MD 21218, USA       email   hutter@jhuvms.hcf.jhu.edu



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