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From: Huitman Huang <huihuang@iupui.edu>
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Subject: a question
Date: Wed, 18 Apr 2001 14:14:04 -0500
Organization: Indiana University - Purdue University Indianapolis,IN
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Dear All,

  After aminoethylation of polyacrylamide(bio-gel p100), 
I wash 1g this amine-containing matrix with water and then with 0.1M 
sodium phosphate, 0.15M NaCl, pH 7.0 (coupling buffer). Drain to a 
moist cake.And then add 100ml 12.5% glutaraldehyde (w/v) in coupling
buffer to the gel. Again add 0.6 gm sodium cyanoborohydride (toxic; 
use a fume hood) or 0.5g sodium borohydride to the gel and mix for 
at least 4hr. But when I add glutaraldehyde to gel(matrix),the gel'colour 
change from white to yellow immediately,and become more and more deeper, 
become brownish black at the end of reaction.

Is it normal?

Thanks in advance!


.