From owner-biophys@hgmp.mrc.ac.uk Wed Oct 10 19:40:44 2001 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 213FB17AA5 for ; Wed, 10 Oct 2001 19:40:44 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 4D7C217AAE for ; Wed, 10 Oct 2001 19:40:41 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8751417AA5 for ; Wed, 10 Oct 2001 19:40:37 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id TAA14158 for biophys-list@hgmp.mrc.ac.uk; Wed, 10 Oct 2001 19:34:18 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Austin So (Hae-Jin)" X-Newsgroups: bionet.biophysics Subject: Re: reference on the melting of broken DNA Date: Wed, 10 Oct 2001 11:33:56 -0700 Organization: ITServices, University of British Columbia Lines: 44 Message-ID: <9q24a4$skk$1@nntp.itservices.ubc.ca> References: X-Complaints-To: abuse@interchange.ubc.ca X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.50.4807.1700 X-MimeOLE: Produced By Microsoft MimeOLE V5.50.4807.1700 To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk "Guosheng Wu" wrote in message news:Pine.GSO.4.33.0110101140050.12014-100000@mercury.chem.nwu.edu... > Any similar experimental or simulation results available? I know haven't seen anything about this kind of system...but I haven't been looking in this particular direction, so it may be out there. > G-G-G-G-G-G-G-G-G-G-A-A-A-A-A-A-A-A-A-A > C-C-C-C-C-C-C-C-C-C T-T-T-T-T-T-T-T-T-T > As mentioned before, 20 base-pair sequence, but there is no connection > between the last base C and the 1st base T. Presumely, the melting will > be determined by the 1st half of the sequence, since it's more stable, but > will it be possible to have two peaks on the derivative of the melting > curve? I'm pretty sure you would get two transitions, and I bet it would be more apparent in the DSC than via optical methods because it is a "polymer". And the reason you would see the distinction is the length of the oligomers are fairly short. That being said, and as far as references are concerned, Breslauer has recent evidence to suggest that order in the solute (in this case your DNA) would be propagated through the solvent to influence the melting behaviour of the other species. -- --- Austin P. So (Hae Jin) I.I.S.G.P. Biotechnology Laboratory University of British Columbia E-mail: haejin@netinfo.ubc.ca http://server1.biotech.ubc.ca/~austin/ .