From owner-cellbiol@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!PILOT.MSU.EDU!delgadoi
From: delgadoi@PILOT.MSU.EDU ("Ivan J Delgado")
Newsgroups: bionet.cellbiol
Subject: glycosylation
Date: 3 Feb 1997 13:02:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <199702032102.QAA19223@pilot19.cl.msu.edu>
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    A question regarding glycosylation of proteins and how that looks in a
western. I have two different antibodies raised against the same protein. When
I use these on a western, in one case I get a tight band while (antiboby 1) and
in the other I get a smear of smaller molecular weight bands (antibody 2). I
don't think it is due to degradation since the first antibody gives a clean
signal (protein sample was the same for both and the same wetsern). Could this
be glycosylation?. How could one test for it?
    Ivan



________________________________________________________________________________


Ivan Delgado
DOE Plant Research Laboratory
Department of Genetics
Michigan State University
East Lansing, MI 48824
office: (517)353-3519 (Dr. N. Raikhel)
FAX: (517)353-9168
delgadoi@pilot.msu.edu




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