From owner-drosophila@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!news.sprintlink.net!demon!doc.news.pipex.net!pipex!lyra.csx.cam.ac.uk!mole.bio.cam.ac.uk!sr120 From: sr120@mole.bio.cam.ac.uk (Steven Russell (Genetics)) Newsgroups: bionet.drosophila Subject: quantitative RT-PCR Date: 15 May 1995 08:11:54 GMT Organization: University of Cambridge, England Lines: 15 Message-ID: <3p72ca$53r@lyra.csx.cam.ac.uk> NNTP-Posting-Host: mole.bio.cam.ac.uk Summary: quantitative RT-PCR Keywords: quantitative RT-PCR The best description of quantitative RT-PCR i have come accross is to be found in Huet et al Development 118 p613 (1993) and Development 121 p1195 (1995). Francois has established conditions for PCRing from as little as a fiftieth of a single salivary gland. His conditions and primers are all described in the first paper. Best wishes Steve Russell Dept of Genetics University of Cambridge UK SR120@mole.bio.cam.ac.uk .