From owner-dros@hgmp.mrc.ac.uk Sun Feb 6 20:57:15 2005 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 1CED77D0C4 for ; Sun, 6 Feb 2005 20:57:15 +0000 (GMT) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 127B97D0E7 for ; Sun, 6 Feb 2005 20:57:14 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 975E37D0E2; Sun, 6 Feb 2005 20:57:04 +0000 (GMT) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id E47A87D129 for ; Fri, 4 Feb 2005 22:16:34 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 5DE707D120; Fri, 4 Feb 2005 22:16:32 +0000 (GMT) To: bionet-drosophila@net.bio.net From: glaser@wadsworth.org (Robert Glaser) Newsgroups: bionet.drosophila Subject: EMCV IRES in flies Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" ; format="flowed" X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 88BA07D124 for ; Fri, 4 Feb 2005 22:16:31 +0000 (GMT) X-Received: from csserv.wadsworth.org (csserv.wadsworth.org [199.184.18.82]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B90147D120 for ; Fri, 4 Feb 2005 22:16:26 +0000 (GMT) X-Received: from [10.63.19.62] (cmsf5-62 [10.63.19.62]) by csserv.wadsworth.org (8.11.7p1+Sun/8.11.7) with ESMTP id j14MGA602073 for ; Fri, 4 Feb 2005 17:16:20 -0500 (EST) X-Sender: glaser@mailhost.wadsworth.org X-To: dros@net.bio.net X-Wadsworth-MailScanner-Information: Please contact CSS for more information X-Wadsworth-MailScanner: Found to be clean X-MailScanner-From: glaser@wadsworth.org Date: Sun, 6 Feb 2005 20:57:04 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk I'm aware that the EMCV IRES works very poorly in insect cells in general. Nonetheless, has anyone used the EMCV IRES in transgenic flies and knows how well is does or doesn't actually work in vivo? Thanks. -- Robert L. Glaser, Ph.D. Wadsworth Center, Div. Genetic Disorders New York State Department of Health & University at Albany, State University of New York P. O. Box 22002 Albany, NY 12201-2002 phone: 518-473-4201 fax: 518-474-3181 e-mail: glaser@wadsworth.org www.wadsworth.org/resnres/bios/glaser.htm --- .