From owner-cellbiol@net.bio.net Wed Jan 29 22:00:00 1997
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From: a8803349@unet.univie.ac.at (Martin Offterdinger)
Newsgroups: bionet.cellbiol
Subject: Re: natural killers
Date: Thu, 30 Jan 1997 08:45:42 GMT
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On 27 Jan 1997 23:00:35 GMT, psittcsis@aol.com (Psittcsis) wrote:

>Can anyone provide me with a reference for a Natural Killer cell
>functional assay?  I can isolate them on the FACS, but cant determine
>their functional status...
The most commonly used assay for NK activity is Cr-31-release assay :
Briefly target cells for NK cells (if you use human NK cells you can
use the leukemia cell line K562) are loaded with radioactive Cr 31, Nk
cells are mixed with target cells at different ratios and cells are
centrifuged off and radioactivity in the supernatant is determined
-include positive control: lysis with 1% SDS and negative control: no
NK cells(= spontenous relaese)-this will yield you are curve: x-values
are the NK:target ratios Y are % release of Cr 31
There are many references in literature about this assay-the
alternative would be using Europium(?) and a fluorescent detection ,
but the Cr assay is still more widely used.

Hope this helps Martin


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