From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!GENETICS.BIO-RAD.COM!bwallace From: bwallace@GENETICS.BIO-RAD.COM (Bruce Wallace) Newsgroups: bionet.diagnostics Subject: Re: sample collection Date: 13 Apr 1995 08:36:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >In article <1FA3363D96@mercury.uark.edu>, DRHOADS@MERCURY.UARK.EDU >("Douglas Rhoads") says: >> >>I would like to poll the this newsgroup as to what methods others >>might recommend for sample collection. We need to collect microliter >>amounts of blood for PCR analysis. Unfortunately, the samples should >>be most easily collected by untrained animal handlers. We have >>looked into cap tubes and the micro-vacutainer type collection tubes >>but all of these require using a lancet and then collection from skin >>surfaces. It would be really nice if there was a vacutainer/needle >>system that collects only 10-100 microliters but those systems start >>at around 2 ml. Since we are using PCR we are very concerned that >>with surface collection there can be cross-contamination from >>handlers hands and from dander on the skin surface. We also don't >>want to have to design something. >> >>Does anyone have any suggestions where we might look?? >> Doug, Blood is routinely collected from infants by puncturing the heel with a lancet, blotting the blood onto filter paper (S&S 903) and drying the specimen. This method of collecting blood has been shown to be adequate for use in DNA probe analysis including PCR and LCR. More importantly, there does not seem to be a problem of cross contamination when filter papers containing dried blood spots from different individuals are stored in physical contact with each other. Bruce Wallace --/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/-- Bruce Wallace, Molecular Diagnostics Tel: 510-741-6532 Bio-Rad Laboratories Fax: 510-741-1051 2000 Alfred Nobel Dr. Hercules CA 94547 email: bwallace@genetics.bio-rad.com .