From owner-celegans@hgmp.mrc.ac.uk Mon Apr 17 18:00:25 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 928A917B23; Mon, 17 Apr 2000 18:00:18 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 9751D17B16; Mon, 17 Apr 2000 18:00:09 +0100 (BST) To: celegans@net.bio.net Newsgroups: bionet.celegans Date: Mon, 17 Apr 2000 16:31:29 +0100 From: slinden@niob.knaw.nl (Sander van der Linden) Subject: integration with UV-irradiation Organization: Hubrecht Laboratory Message-Id: <20000417170009.9751D17B16@mercury.hgmp.mrc.ac.uk> Sender: owner-celegans@hgmp.mrc.ac.uk Precedence: bulk Hi all, A frequently asked question? I like to use UV-irradiation for integration of extrachromosomal arrays. I would be grateful if anyone could send me a reply with answers on the following questions: a) energy dose (µJ x 100) used with a Stratagene UV Stratalinker 1800. (or even better, what settings do you find work best for the Stratagene Stratalinker?) b) frequency of integrants per F1. kind regards, Sander van der Linden Hubrecht Laboratory Uppsalalaan 8 3584 CT Utrecht The Netherlands slinden@niob.knaw.nl --- .