From owner-drosophila@net.bio.net Mon Jun 10 23:00:00 1996 Path: biosci!rutgers!uwm.edu!chi-news.cic.net!condor.acc.iit.edu!not-for-mail From: garfinkl@charlie.cns.iit.edu (Mark D. Garfinkel) Newsgroups: bionet.drosophila Subject: Re: Anyone microinject linear DNA? Date: 11 Jun 1996 12:28:30 -0500 Organization: Illinois Institute of Technology Lines: 14 Message-ID: <4pkabu$okr@charlie.cns.iit.edu> References: <4phnp9$1ss@taco.cc.ncsu.edu> NNTP-Posting-Host: charlie.cns.iit.edu In article <4phnp9$1ss@taco.cc.ncsu.edu>, Dipak Mahato wrote: >Hi Everyone, > >I was wondering if anyone has every tried to make transgenics flies using >linearized plasmid, as opposed to circular. Yes. It doesn't work. You might want to spend some time in a library reading classic papers on the topic. Mark -- Mark D. Garfinkel (e-mail: garfinkl@charlie.acc.iit.edu) (c) 1996; all rights reserved. Permission granted for Usenet quotation with attribution. .