From owner-cellbiol@net.bio.net Tue Feb 25 22:00:00 1997 Path: biosci!ucdavis.edu!jzsmith From: jzsmith@ucdavis.edu (John Smith) Newsgroups: bionet.cellbiol Subject: stable transfection of suspension cells Date: 26 Feb 1997 13:00:46 -0800 Organization: Ceprap Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <3314A46C.4D56@ucdavis.edu> Reply-To: jzsmith@ucdavis.edu NNTP-Posting-Host: net.bio.net I am looking for a detailed protocol for the stable transfection of mouse T cell hybridoma suspension cells, in particular the selection part (in this case using G418). Any help welcome. Thanks, Bert Overduin (bboverduin@ucdavis.edu) .