From owner-celegans@net.bio.net Tue Aug 18 23:00:00 1998
Path: biosci!biosci!not-for-mail
From: patterson@waksman.Rutgers.EDU (Garth Patterson)
Newsgroups: bionet.celegans
Subject: RE: GFP antibody staining
Date: 19 Aug 1998 12:16:00 -0700
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Sascha,

In Gary Ruvkun's lab, I used the clontech rabbit anti-GFP polyclonal
antibody quite a bit, and it never gave me the problem you describe.  I
found that the GFP I saw in fixed worms with the antibody matched the GFP I
saw directly in live worms.  This is using the mutated GFP from the Andy
Fire kit--the one with a change in residue 65.  I have no experience with
the souped-up clontech GFP you describe.

Garth

Garth I. Patterson
Department of Molecular Biology
Rutgers University

Temporary address and phone (through Sept. 18, 1998)

Center for Advanced Biotechnology and Medicine
Room 102
675 Hoes Lane
Piscataway, NJ 08854

Ph. 732-235-5331
Fax 732-235-4880



.