From owner-celegans@net.bio.net Wed Oct 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Tritech_Research@lamg.com Newsgroups: bionet.celegans Subject: inject worms w/cheap mineral oil Date: 16 Oct 1997 11:02:59 -0700 Organization: Los Angeles Macintosh Group Lines: 56 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <625ksj$she@net.bio.net> NNTP-Posting-Host: net.bio.net Several more people confirm that mineral oil is the way to go. So, save money and use it instead. I want to start putting a list of money-saving tips like this into our new website, which is, for the time being, at: http://members.aol.com/TriResInc (Yes, I am embarrassed, and yes, AOL sucks) So, please contribute other tips. There is a way to do that from the site, too. Here is more support for using the mineral oil instead of halocarbon... Andy tritech_research@LAMG.COM ----------------------- I used halocarbon oil in Boulder and Mineral oil when I moved to SF. It's all we use now. Craig P. Hunter ----------------------- Hi Andy, I've been using light mineral oil from Sigma for my micro-injections with reasonable results. I'm not a great micro-injector, so I can't say that it works just as well as halocarbon oil or not, but I've been able to get transgenics with no obvious problems from the oil. I'm pretty good at killing worms, though... John Lye ----------------------- Andy- Of course, the dechorionated fly eggs are stuck onto doublestick tape and submerged in the halocarbon oil for 24 during development- we rescue our adult worms immediately... Anne C. Hart ----------------------- Hi ANdy- We use mineral oil as well. Worms dry out more quickly with mineral oil, but I prefer it: not as messy to work with. To deal with the drying out problem, I tend to inject one worm at a time, but have found that I can inject as quickly as people lining up multiple worms on a slide. And the worms survive better, seem healthier if I only inject one at a time. All the best, Susan. Susan Mango ----------------------- Hi Andy, Our lab has always used mineral oil (Sigma) for injections. I can't remember off-hand if it is heavy or light (I think heavy). Paul Paul Muhlrad ----------------------- So - there you have it.... -AP .