From owner-cellbiol@net.bio.net Tue Feb 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!howland.erols.net!news.nacamar.de!uni-erlangen.de!winx03!news From: Christian Ammon Newsgroups: bionet.cellbiol Subject: Re: vital dye in the visible range Date: Wed, 26 Feb 1997 11:03:22 -0800 Organization: HNO-Klinikum Lines: 17 Message-ID: <331488FA.10C5@mail.uni-wuerzburg.de> References: NNTP-Posting-Host: 132.187.128.103 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01E -NOV-NOV (Win16; I) Kiran Bhadriraju wrote: > > Hi! > > I am in search of a dye to stain living cells which would absorb in the > in the visible range. I looking at hepatocytes through an inverted > microscope (attached to an atomic force microscope, but that is > irrelevent) and they don't show very well at all. The cells are on a > glass cover slip stuck to a steel AFM specimen stub. So my idea is to > use a dye in conjunction with a white background for the coverslip. I cant help you with the dye, but are you already using Nomarski Contrast? christian ammon .