From owner-cellbiol@net.bio.net Tue Jan 28 22:00:00 1997
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From: Jim Wu <jim_wu@berlex.com>
Newsgroups: bionet.cellbiol
Subject: PDGF antibodies?
Date: Tue, 28 Jan 1997 17:04:53 -0800
Organization: Berlex Labs
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CC: bionet.neuroscience

I am looking for PDGF antibodies, preferably reactive against native,
human material, which I assume must cover all of the isoforms:  AA,
AB, and BB.  In addition, it is important that this antibody can be
used for:

1)  neutalising activity in proliferative assays

2)  coupling to a column gel for removing PDGF from cell supernatants

I have tried R&D Systems polyclonal #AB-23-NA in my bioassay, and it
did not appear to neutralise activity under my particular conditions
at a concentration of 10ug/ml  (ab was preincubated with std for 2 hrs
and compared to an IgG control).  It does work well, however, in
Western blots.

Please email me, if you have any suggestions.

thanks in advance,

jim_wu@berlex.com

.