From owner-diagnostics@net.bio.net Mon Mar 20 22:00:00 1995
Path: biosci!ZEUS.DATASRV.CO.IL!ila2027
From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish)
Newsgroups: bionet.diagnostics
Subject: Re: Mabs from PAGE bands
Date: 20 Mar 1995 20:20:12 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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On 16 Mar 1995, Robert Burns wrote:

> Hi all,
>      my first posting to the "now permanent" diagnost list
> 
> My question: Has anybody raised Mabs using protein bands excised
> from PAGE gels as the immunogen? If so can you use the gel band
> itself as an implant without having to elute the protein?
> 
> Second question ( I have asked this before but for the benefit of
> newcomers) Is anybody interested in Fungal diagnostics by serological
> methods?
> 
> 
> Robert
> 
> 
> 
> 
> 
> Robert Burns
> 
> Monoclonal Antibody Unit
> Scottish Agricultural Science Agency
> East Craigs
> Edinburgh
> Scotland
> 
> burns@sasa.gov.uk
> 
> 
> 
> 
Yes, you could immunize with the gel without elution, but macerate 
(grind) it first to smaller particles.

Falk Fish.


.