From owner-cellbiol@net.bio.net Tue Feb 25 22:00:00 1997
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From: divernlb@aol.com (DiverNLB)
Newsgroups: bionet.cellbiol
Subject: Problem with nonspecific antibody binding
Date: 26 Feb 1997 00:31:15 GMT
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Hello. I am currently developing a protocol for immunofluorescent
detection of the stress proteins hsp70 and cpn60 on cryosectioned Mytilus
mantle tissue. The problem I am having is nonspecific binding of the
secondary antibody (conjugated to fluorescein), leading to strong
false-positive signals. I have tried blocking with bovine serum albumin,
fetal bovine serum, and horse serum, with little or no effect. Could
anyone suggest any other solutions? Please respond to DiverNLB@aol.com.
Thank you.


Homer O. Ellison
Dept. of Biological Sciences
California State University, Long Beach


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