From owner-drosophila@net.bio.net Mon Dec 13 22:00:00 1993
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!uunet!sangam!vikram!imtech!bic_ccmb
From: bic_ccmb@imtech.ernet.in
Newsgroups: bionet.drosophila
Subject: melanogaster salivary glands
Message-ID: <8277@imtech.ernet.in>
Date: 11 Dec 93 22:51:47 +0530
Distribution: world
Organization: Inst. of Microbial Technology, Chandigarh, India
Lines: 24

From indirect evidences, I suspect larval salivary gland-specific DNA
rearrangement to have taken place in D. melanogaster. The rearrangement
has been seen w. r. t. an X-chromosome specific repetitive DNA probe. So,
I need to do Southern hybridization of embryonic vs. salivary gland
DNA(from third instar larvae), with this probe, to be comletely sure
of the rearrangement. 
Ashburner's lab manual (referring Cohen and Gotchell, 1971)gives a method
using a specific kind of pestle and a very finely roughened homogenizing
tube. It is impossible for me to get anything, with that kind of
specifications, made here. If anyone has seen any other method, can write
the method to me or let me know the reference. Otherwise any unpublished,
working protocol will also be of great help and will be duely acknowledged. If
anyone can donate me that special kind of pestle, used by Cohen and Gotchell,
it will be highly appreciated.
Thanking you in advance for any kind of help.
SANJAY
Research Fellow
CCMB
My e-mail address is:
lalji%ccmb@uunet.in 

             
             


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