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From: Mary McCarthy <mmccarthy@BioTechniques.com>
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Subject: BioTechniques 24(5), May 1998
Date: 17 Apr 1998 08:56:04 -0700
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BioTechniques 24(5), May 1998
Table of Contents

Benchmarks

Construct for Assessment of Transfected Secretory Proteins Using an
Independently Secreted
Reporter Gene
     M. Ailenberg and M. Silverman

On-Line Microdialysis for Mass Spectrometry
     S. Konig, S. Weiler and H.M. Fales

Single-Step Purification of T7 RNA Polymerase with a 6-Histidine Tag
     T. Ellinger and R. Ehricht

Differential mRNA Display at the Single-Cell Level
     C. Renner, L. Trumper, J.-P. Pfitzenmeier, U. Loftin, K. Gerlach, I.
Stehle, 
     A. Wadle and M. Pfreundschuh

Simplified Method for the Telomeric Repeat Amplification Protocol (TRAP)
     L.N. Sellner, P.M. Lock and G.R. Turbett

Artifact Occurring during Combined In Situ Hybridization and
Immunocytochemistry Analysis
because of Positive Chemography
     T. Hakkinen and S. Yla-Herttuala

DNase I Treatment of Total RNA Improves the Accuracy of 
Ribonuclease Protection Assay
     D.A. Dixon, D.L. Vaitkus and S.M. Prescott

Fusion PCR, a One-Step Variant of the Megaprimer Method 
of Mutagenesis
     C. Karreman

Real-Time Fluorescence Detection of a Single DNA Molecule
     C. Lockey, E. Otto and Z. Long

One-Step Procedure for Screening Recombinant Plasmids by Size
     E. Beuken, C. Vink and C.A. Bruggeman

Enhanced Efficiency of Cloning FACS-Sorted Mammalian Cells
     C. Rodriguez and H.F. Lodish

Isolation of Stable Bacterial Artificial Chromosome DNA Using a 
Modified Alkaline Lysis Method
     D. Sinnett, C. Richer and A. Baccichet

Rapid Zymogram for Lipase
     R.P. Yadav, R.K. Saxena, R. Gupta and W.S. Davidson

Modified Hirt Procedure for Rapid Purification of Extrachromosomal DNA from
Mammalian
Cells
     U. Arad

Concentrating Dilute Protein Solutions for Gel Electrophoresis
     S. Yitzhaki and J. Sperling

Minimizing False Positives in Differential Display
M.J. Luce and P.D. Burrows


Internet On-Ramp
     Cyberspace for Biologists


Short Technical Reports

Vectors for Expressing T7 Epitope- and His6 Affinity-Tagged Fusion Proteins
in S. cerevisiae
     S. Enomoto, G. Chen and J. Berman

UGA Read-Through Artifacts - When Popular Gene Expression 
Systems Need a pATCH
     G. MacBeath and P. Kast

Microsatellite Enrichment in Organisms with Large Genomes 
(Allium cepa L.)
     D. Fischer and K. Bachmann

Multiwell 14CO2-Capture Assay for Evaluation of Substrate 
Oxidation Rates of Cells in Culture
     C.L. Collins, B.P. Bode, W.W. Souba and S.F. Abcouwer


Diagnostic Techniques

Quantitative, Competitive PCR Assay for HIV-1 Using a 
Microplate-Based Detection System
     P.C. Guenthner and C.E. Hart

Fast-Painting of Human Metaphase Spreads Using a Chromosome-Specific,
Repeat-Depleted
DNA Library Probe
     M. Durm, L. Schussler, H. Munch, J. Craig, H. Ludwig, M. Hausmann 
     and C. Cremer

Quick-FISH: A Rapid Fluorescence In Situ Hybridization Technique 
for Molecular Cytogenetic Analysis
     S.K. Banerjee, A.P. Weston, D.L. Persons and D.R. Campbell

High-Throughput Screening for Known Mutations by Automated 
Analysis of Single Sequencing Reactions
     T. Kristensen, V.N. Kristensen and A.-L. Borresen-Dale

Detection of Single-Nucleotide Polymorphism
     W. Gu, G.D. Aguirre and K. Ray

Doublex Sequencing in Molecular Diagnosis of Hereditary 
Diseases
     J. Plaschke, H. Voss, M. Hahn, W. Ansorge and H.K. Schackert


Research Reports

3-prime-End cDNA Pool Suitable for Differential Display from a Small 
Number of Cells
     S. Zhao, G. Molnar, J. Zhang, L. Zheng, L. Averboukh and A.B. Pardee

Development of Competitive mRNA PCR for the Quantification of
Interleukin-6-Responsive junB
Oncogene Expression
     P. Igaz, G. Fejer, C. Szalai, S. Toth and A. Falus

E. coli-Based In Vitro Transcription/Translation: In Vivo-Specific
Synthesis Rates and High
Yields in a Batch System
     R. Patnaik and J.R. Swartz

Functional Assays to Identify and Characterize Regulators of 
Microtubule Behavior
     J. McMeekan and P. Wadsworth

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