From owner-celegans@net.bio.net Sun Jan 14 22:00:00 1996
Path: biosci!MERCK.BCH.UMONTREAL.CA!labbej
From: labbej@MERCK.BCH.UMONTREAL.CA (Jean-Claude LABBE)
Newsgroups: bionet.celegans
Subject: Array integration
Date: 15 Jan 1996 15:06:58 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <Pine.3.88.9601151732.A26762-0100000@merck.BCH.UMontreal.CA>
Reply-To: Jean-Claude LABBE <labbej@merck.BCH.UMontreal.CA>
NNTP-Posting-Host: net.bio.net

Dear Breeders,

	I am trying to integrate an extrachromosomal array that I 
injected into a chromosome of the N2 strain.  I used the dominant marker 
pRF4 for the selection.  I followed the protocol published by S. Mitani 
(WBG 14, p.22) using UV as the mutagenic source, but it  failed to give 
any integrants (I screened 500 F1s and 2 F2s for each F1).  Could anybody 
give me some advice on how to perform integration.

Thanks,

Jean-Claude Labbe
Departement de biochimie
Universite de Montreal

.