From owner-celegans@hgmp.mrc.ac.uk Tue Oct 24 22:10:43 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8FAE917B97; Tue, 24 Oct 2000 22:10:42 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 7A5DD17B9E; Tue, 24 Oct 2000 22:10:41 +0100 (BST) To: celegans@net.bio.net Newsgroups: bionet.celegans Date: Mon, 23 Oct 2000 14:25:16 +0100 From: chaser@geguri.kjist.ac.kr ("Kyuyeong, Choi") Subject: The sequence of pRF4 Organization: K-JIST Message-Id: <20001024211041.7A5DD17B9E@mercury.hgmp.mrc.ac.uk> Sender: owner-celegans@hgmp.mrc.ac.uk Precedence: bulk Dear breeders, I have conducting microinjections with pRF4 as a marker. I have pRF4 plasmid, but I don't know the sequence or enzyme mapping data. If you know the information about pRF4, would you tell me it? Another question ; I had F1 roller, but failed to F2 roller pogeny. How do I get roller transgenics? Kyuyeong, Choi from South Korea chaser@geguri.kjist.ac.kr --- .