From owner-celegans@net.bio.net Mon Dec 09 22:00:00 1996 Path: biosci!LIFESCI.LSCF.UCSB.EDU!gendreau From: gendreau@LIFESCI.LSCF.UCSB.EDU (Steve Gendreau) Newsgroups: bionet.celegans Subject: pharynx markers Date: 10 Dec 1996 10:25:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Would anyone have a transgenic strain that expresses a reporter fusion construct in some or all of the nuclei in the embryonic pharynx? I have a zygotic embryonic lethal that may make extra pharyngeal cells and would like to test my suspicion. I have tried staining with various non-nuclear localized pharynx-specific antibodies (3NB12 and 9.2.1) and have not been successful counting the number of immunoreactive cells in the unhatched monsters. Thanks for your help. Steve Steve Gendreau Neuroscience Research Institute University of California Santa Barbara, CA 93106 (805) 893-8090 phone (805) 893-4724 fax gendreau@lifesci.lscf.uscb.edu .