From owner-dros@hgmp.mrc.ac.uk Tue Aug 6 15:20:38 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 6DD0E17C72 for ; Tue, 6 Aug 2002 15:20:34 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 7A2D217C86 for ; Tue, 6 Aug 2002 15:20:29 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id E93C717C45; Tue, 6 Aug 2002 15:20:07 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1AC3417A8F for ; Tue, 6 Aug 2002 01:49:09 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 7AF1F17A80; Tue, 6 Aug 2002 01:49:07 +0100 (BST) To: bionet-drosophila@net.bio.net From: greg_lamonte@hotmail.com ("Greg LaMonte") Newsgroups: bionet.drosophila Subject: (NONE) Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: Mime-Version: 1.0 Content-Type: text/plain; format=flowed X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DC0D017A8F for ; Tue, 6 Aug 2002 01:49:02 +0100 (BST) X-Received: from hotmail.com (f210.pav2.hotmail.com [64.4.37.210]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 38A1317A80 for ; Tue, 6 Aug 2002 01:48:55 +0100 (BST) X-Received: from mail pickup service by hotmail.com with Microsoft SMTPSVC; Mon, 5 Aug 2002 17:48:54 -0700 X-Received: from 63.98.212.2 by pv2fd.pav2.hotmail.msn.com with HTTP; Tue, 06 Aug 2002 00:48:53 GMT X-Originating-IP: [63.98.212.2] X-To: dros@net.bio.net X-OriginalArrivalTime: 06 Aug 2002 00:48:54.0100 (UTC) FILETIME=[09E46140:01C23CE3] Date: Tue, 6 Aug 2002 15:20:07 +0100 (BST) X-Razor-id: 3af82c28b6c263636334cde4f15fac523d7adb38 Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hello, I've been working with larval extracts, and my samples have a yellow/brown color. I tried filtering the color away with a spin filtration tube (4k size), but it didn't get rid of it. The reason this coloration is a problem is that I believe the same substance that causes this coloration also collets at the top of the gel filtration column when the extract is run through said columns. I am hoping someone can identify what might be causing the coloration, and how to get rid of it in subsequent larval preparations. Any help would be appreciated. -Greg LaMonte _________________________________________________________________ Send and receive Hotmail on your mobile device: http://mobile.msn.com --- .