From owner-cellbiol@net.bio.net Wed Dec 25 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!ix.netcom.com!news From: Neal Machtiger Newsgroups: bionet.cellbiol,bionet.general,bionet.metabolic-reg,bionet.microbiology,bionet.molbio.methds-reagnts,bionet.molbio.yeast Subject: Re: phospholipid separation method Date: Thu, 26 Dec 1996 11:47:13 -0800 Organization: Netcom Lines: 6 Message-ID: <32C2D641.32B4@ix.netcom.com> References: <851618393.22572@dejanews.com> Reply-To: neal_m@ix.netcom.com NNTP-Posting-Host: nbw-nj10-01.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Thu Dec 26 10:41:03 AM PST 1996 X-Mailer: Mozilla 3.01GoldC-nnie30 (Win16; U) Xref: biosci bionet.cellbiol:6281 bionet.general:24651 bionet.metabolic-reg:907 bionet.microbiology:8232 bionet.molbio.methds-reagnts:52884 bionet.molbio.yeast:6301 TLC has been used to separated Phosopolipids for 30+ years. The solvent systems were Chloform/Methanol/water. Dont know if CHCL3 has been replaced by something less toxic. Neal .