From owner-celegans@net.bio.net Thu Jun 13 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!news.uoregon.edu!arclight.uoregon.edu!news.bc.net!rover.ucs.ualberta.ca!elegans.biology.ualberta.ca!user From: Morris_maduro@biology.ualberta.ca (Morris Maduro) Newsgroups: bionet.celegans Subject: imipramine and egg laying Followup-To: bionet.celegans Date: 14 Jun 1996 17:45:34 GMT Organization: Genetics U of Alberta Lines: 13 Message-ID: NNTP-Posting-Host: elegans.biology.ualberta.ca Dear Worm Folks, I am having trouble using imipramine to stimulate egg laying even in N2 controls (I'm trying to test unc-119 mutants). I have made a 0.75 mg/mL solution in M9 of imipramine hydrochloride (Sigma I-7379) and even after one hour in the solution there is no evidence of egg laying although the animals survive the treatment quite readily. Treatment with serotonin (10 mg/mL) works fine, so what could be going on here? Morris morris_maduro@biology.ualberta.ca .