From owner-drosophila@net.bio.net Tue Oct 12 23:00:00 1993 Path: biosci!rutgers!netnews.upenn.edu!gradmac.bio.upenn.edu!user From: kcrossgr@mail.sas.upenn.edu (Kirsten Crossgrove) Newsgroups: bionet.drosophila Subject: p element question Message-ID: Date: 13 Oct 93 19:48:16 GMT Sender: news@netnews.upenn.edu Followup-To: bionet.drosophila Organization: University of Pennsylvania Lines: 11 Nntp-Posting-Host: gradmac.bio.upenn.edu Hi, I am about to try P-element mediated transformation for the first time. I am using a pCaSpeR construct with the hsp70 promoter and 3'trailer. The cDNA I cloned in has about 560 bp upstream of the ATG and it has 11 C's at the 5'most end (artifact of the original cDNA cloning process). Is there any reason I should get rid of either the polyC region or the whole upstream 560bp in terms of getting good expression of the transgene? I'd appreciate any advice. Thanks, Kirsten Crossgrove kcrossgr@mail.sas.upenn.edu .