From owner-drosophila@net.bio.net Thu Oct 06 23:00:00 1994 Path: biosci!PUCC.PRINCETON.EDU!A910741%JPNTMU00.BITNET From: A910741%JPNTMU00.BITNET@PUCC.PRINCETON.EDU ("Y. Fuyama") Newsgroups: bionet.drosophila Subject: Re: Anesthetic? Date: 7 Oct 1994 01:52:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <199410070852.BAA24047@net.bio.net> NNTP-Posting-Host: net.bio.net On 6 Oct 1994 23:02:36 GMT Scott Panzer said: > There's also something called 'Fly-Nap' that is fairly long lasting. > I think companies that supply school labs with flies (eg Carolina) > have this stuff. I don't know what chemical it actually is. I understand that the effective component of Fly-Nap (TM) is triethylamine. We use a conventional plastic wash bottle as an anesthetizer using triethylamine. Put cotton wad in it and pour small amount (ca 1 ml) of triethyamine. (This amount is enough to use for a whole day or even a few days!) Blow evaporated gas into a vial containing flies and wait until they stop moving. Be sure that the flies stay at the bottom of the vial because triethylamine is much heavier than air. Anesthesia by triethylamine lasts at least 30 min, and often over 1 hr. (Our students used to finish chi-square tests before flies wake up!) --- Yoshiaki FUYAMA :Dept. of Biology, Tokyo Metropolitan Univ. A910741@JPNTMU00.BITNET yfuyama@yamabuki.comp.metro-u.ac.jp (Internet) .