From owner-dros@hgmp.mrc.ac.uk Sun Sep 19 16:03:25 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 888587D0C5 for ; Sun, 19 Sep 2004 16:03:25 +0100 (BST) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0EB037D0CE for ; Sun, 19 Sep 2004 16:03:25 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id E21397D0C5; Sun, 19 Sep 2004 16:03:21 +0100 (BST) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A60F27D117 for ; Fri, 17 Sep 2004 12:18:10 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 739227D0C9; Fri, 17 Sep 2004 12:18:09 +0100 (BST) To: bionet-drosophila@net.bio.net From: Man.Mohan@gen.bio.uni-giessen.de (Man Mohan) Newsgroups: bionet.drosophila Subject: stable integration of puast in S2 Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research Message-ID: <20040917110242.EF05D2D2DF6@hermes2.hrz.uni-giessen.de> Content-Transfer-Encoding: 8bit Content-Type: text/plain; charset=iso-8859-1 MIME-Version: 1.0 X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 350417D115 for ; Fri, 17 Sep 2004 12:18:08 +0100 (BST) X-Received: from merkurneu.hrz.uni-giessen.de (merkurneu.hrz.uni-giessen.de [134.176.2.3]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C939E7D0C9 for ; Fri, 17 Sep 2004 12:18:05 +0100 (BST) X-Received: from [134.176.2.168] by merkurneu.hrz.uni-giessen.de with ESMTP for dros@net.bio.net; Fri, 17 Sep 2004 13:02:45 +0200 X-Received: from popg.uni-giessen.de (jobim.hrz.uni-giessen.de [134.176.3.137]) by hermes2.hrz.uni-giessen.de (Postfix) with ESMTP id EF05D2D2DF6 for ; Fri, 17 Sep 2004 13:02:42 +0200 (CEST) X-User-Agent: IMHO/0.98.3 (Webmail for Roxen) X-To: dros@net.bio.net X-HRZ-JLUG-MailScanner-Information: Passed JLUG virus check X-HRZ-JLUG-MailScanner: Found to be clean Date: Sun, 19 Sep 2004 16:03:21 +0100 (BST) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi, I am planning to stably integrate pUAST (haveing my gene contruct) into S2 cell line. Does anyone know whether it is possible, and how to select. I can try other Drosphila cell lines if needed. Later I would like to also integrate pMT Gal4 so that I can drive the UAS-construct. Your help will be appreciated. best man mohan Institute for Genetics University of Giessen Heinrich Buff Ring 58 Giessen 35392 Germany --- .