From owner-dros@hgmp.mrc.ac.uk Thu Feb 26 14:21:51 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 33DAB7D274 for ; Thu, 26 Feb 2004 14:21:51 +0000 (GMT) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B49397D17C for ; Thu, 26 Feb 2004 14:21:50 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id A27F47D5B0; Thu, 26 Feb 2004 14:21:45 +0000 (GMT) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9D3EA7D214 for ; Wed, 25 Feb 2004 11:48:15 +0000 (GMT) Received: from chx400.switch.ch (chx400.switch.ch [130.59.10.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 541C57D442 for ; Wed, 25 Feb 2004 11:48:11 +0000 (GMT) Received: from [216.239.37.5] (helo=proxy.google.com) by chx400.switch.ch with esmtp (Exim 3.20 #1) id 1AvwYT-0006Ty-00 for bionet-drosophila@moderators.isc.org; Wed, 25 Feb 2004 11:46:17 +0100 Received: from 10.7.0.80 œby Google Production Router with ESMTPœ id i1PAkEih012332 for ; Wed, 25 Feb 2004 02:46:14 -0800 Received: (from news@localhost) by Google Production with id i1PAkENR022404 for bionet-drosophila@moderators.isc.org; Wed, 25 Feb 2004 02:46:14 -0800 To: bionet-drosophila@moderators.isc.org From: ritz.valentina@mh-hannover.de (Valentina Ritz) Newsgroups: bionet.drosophila Subject: single cell cloning S2 cells Organization: http://groups.google.com Message-ID: <43fee26f.0402250246.6b461279@posting.google.com> Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: posting.google.com 1077705974 22403 127.0.0.1 (25 Feb 2004 10:46:14 GMT) X-Complaints-To: groups-abuse@google.com NNTP-Posting-Date: Wed, 25 Feb 2004 10:46:14 +0000 (UTC) Date: Thu, 26 Feb 2004 14:21:45 +0000 (GMT) X-Amavis-Alert: BAD HEADER Non-encoded 8-bit data (char 9C hex) in message header 'Received' Received: from 10.7.0.80\n\t\234by Google Produ... ^ Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi there, I encountered a severe problem in trying to get single cell clones of S2 cells by limiting dilution in 96 well plates. Do you have a reliable protocol for this? Thanks in advance Valentina .